recombinant mouse muc5ac protein  (Cusabio)

Journal: Biomacromolecules

Article Title: Biodegradable nanoparticles meet the bronchial airway barrier: how surface properties affect their interaction with mucus and epithelial cells.

doi: 10.1021/bm201226x

Figure Lengend Snippet: Figure 5. MUC5AC protein content in the cell lysates (a) and apical secretions accumulated in the monolayer washing solutions (b) after exposure to PLGA/CS, PLGA/PVA and PLGA/PF68 NPs. Untreated cells were used as control. Acrolein solution (0.05 mM) was used as a positive control. Data represent mean ± SD (n = 3).

Article Snippet: To quantify the amount of MUC5AC protein in the washing solutions and in the cell lysates, a commercial ELISA kit was used following the indications of the manufacturer (CUSABIO BIOTECH Co., Ltd.).

Techniques: Control, Positive Control

Journal: Biomacromolecules

Article Title: Biodegradable nanoparticles meet the bronchial airway barrier: how surface properties affect their interaction with mucus and epithelial cells.

doi: 10.1021/bm201226x

Figure Lengend Snippet: Figure 6. MUC5AC gene expression in Calu-3 cells after exposure to PLGA/CS, PLGA/PVA, and PLGA/PF68 NPs. Acrolein (0.05 mM) was used as positive control. The data represent the levels of normalized expression in NP-treated Calu-3 cells relative to control (untreated) cells and are reported as mean ± SD (n = 3); *p < 0.05 (acrolein-exposed vs untreated cells).

Article Snippet: To quantify the amount of MUC5AC protein in the washing solutions and in the cell lysates, a commercial ELISA kit was used following the indications of the manufacturer (CUSABIO BIOTECH Co., Ltd.).

Techniques: Gene Expression, Positive Control, Expressing, Control